Cloning and characterization of newly isolated lipase from Enterobacter sp. Bn12
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چکیده
منابع مشابه
Cloning and characterization of newly isolated lipase from Enterobacter sp. Bn12
A mesophilic Enterobacter sp. Bn12 producing an alkaline thermostable lipase was isolated from soil in Tehran, Iran. The lipase gene (ELBn12) was identified from a genomic library. Sequence analysis of the DNA fragment revealed an open reading frame of 879 bp encoding a lipase with a molecular mass of 31.3 kDa. The deduced amino acid sequence showed 96% identity with a lipase of Enterobacter sp...
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BACKGROUND AND OBJECTIVES Lipases are valuable biocatalysts which are widely used in the detergent, food, dairy and pharmaceutical industries. The aims of the present study included the isolation of a lipase-producer from industrial zones and the partial characterization of the enzyme. MATERIALS AND METHODS A number of bacteria were isolated from sites related to the oil industries. An isolat...
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Background: Pectinases are pectin degrading class of enzymes including polygalacturonase (PG), polymethyl galacturonase (PMG), pectate lyase (PEL), and pectin esterase (PE) that are commonly used in processes involving the degradation of plant materials, such as speeding up the extraction of fruit juices. Objectives: A highly methylated pectin degrading bacterium from soil covered with fruit wa...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2014
ISSN: 1517-8382
DOI: 10.1590/s1517-83822014000200042